FIG. 3.

Sensitivity to Calcofluor White of C. albicans Phr1⁺ and Phr1⁻ cells. At a cell density of 5 × 10⁶/ml, 5 μl of a concentrated suspension (lanes 1) of cells grown in YPD at pH 4.5 or 8 and of 10× (lanes 2), 100× (lanes 3), 1,000× (lanes 4), and 10,000× (lanes 5) dilution was spotted on a solid plate of YPD at the same pH. Amounts of CF present in the plate are indicated on the right in micrograms per milliliter.