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Sensitivity of Phr1− cells to nikkomycin Z. Phr1+ (strain CAF3-1) (▪) and Phr1− (strain CAS8) (▴) cells exponentially growing at pH 4.5 or 6.5 in YNB or at pH 7.4 in RPMI 1640 at 25°C were tested for sensitivity to nikkomycin Z by microdilution assay.