TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and description | Source and/or reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | 4 |
| MH1160 | MC4100 ompR101 | 9 |
| LM101 | MC4100 Δ(ompR-envZ)::Kanr | 18 |
| FR247 | MC4100 ompR101 Φ(ompC′-lacZ+)10–25 envZ247 zhf37::Tn10 (λpSG10) | 22 |
| CYL304 | MC4100 ompR101 envZ247 zhf37::Tn10 | This study |
| MS10095 | JM107 zad::Tn10 pcnB80 | 17; M. Singer |
| CYL302 | MH1160 zad::Tn10 pcnB80 | This study |
| CYL303 | LM101 zad::Tn10 pcnB80 | This study |
| CYL305 | CYL304 zad::Tn10 pcnB80 | This study |
| Plasmids | ||
| pACYC177 | 5 | |
| pUC19 | 27 | |
| pLAN701 | ompR+ cloned into pACYC177a | This study |
| pLAN702 | ompRD55E cloned into pACYC177b | This study |
| pLAN801 | ompR+ cloned into pUC19a | This study |
| pLAN802 | ompRD55E cloned into pUC19b | This study |
a
A 1,050-bp EcoRI-HindIII PCR-generated DNA fragment containing the ompR+ sequence from position −127 to +923 from the start point of ompR transcription.
b
A 1,050-bp EcoRI-HindIII PCR-generated DNA fragment containing the ompR+ sequence with the codon at position 55 changed from GAT to GAA to generate the ompRD55E allele.