FIG. 3.

Phenotypes of minC mutants in the presence of MinD in lon⁺ and lon mutant cells. Representative examples are shown; other results are summarized in Table 1. The lon⁺ ΔminCDE hosts were PB114 (λDB164 P_lac-minD) (a to c) and RC3F⁻/pDB211 (P_aadA-minD) (d). The lon ΔminCDE hosts were MS114 (λDB164) (e to g) and MS114/pDB211 (h). The strains contained minC plasmid pJPB120m4 (P_aadA-minC24) (a and e), pJPB120m11 (P_aadA-minC27) (b and f), pMS709 (P_lac-minC28) (c and g), or pJPB120m5 (P_aadA-minC25) (d and h). Cells were grown overnight in the presence of 0.2 mM IPTG (isopropyl-β-d-thiogalactopyranoside) prior to examination by phase-contrast microscopy (2).