Table 2.
Plasmids used in this study.
| Plasmids | Description | Sources |
|---|---|---|
| pAP-B03 | Carring pheAfbr and aroFwt genes, p15A ori with PRPL;kanR | Our laboratory |
| pCDF-aroGfbr-tyrAfbr | pCDFDuet-1:aroGfbr::tyrAfbr, StrR | Our laboratory |
| ptarget | pMB1,aadA,sgRNA-Blank,SpcR | [26] |
| pCas9 | repA101(Ts) kan Pcas-cas9 ParaB-Red lacI Ptrc-sgRNA-pMB1 | [26] |
| pAP01 | pAP with aroGfbr and tyrAfbr, p15A ori with PRPL;kanR | This study |
| pAP11 | pAP with aroGfbr, tyrAfbr, tkt A and ppsA, p15A ori with PRPL;kanR |
This study |
| pAP12 | pAP with aroGfbr, tyrAfbr and aroABC, p15A ori with PRPL;kanR | This study |
| pAP13 | pAP with aroGfbr, tyrAfbr and aroBEL, p15A ori with PRPL;kanR | This study |
| pAP21 | pAP with aroGfbr, tyrAfbr, tktA, ppsA and aroABC, p15A ori with PRPL;kanR | This study |
| pAP22 | pAP with aroGfbr, tyrAfbr, tktA, ppsA and aroBEL, p15A ori with PRPL;kanR | This study |
| pAP31 | pAP with aroGfbr, tyrAfbr, tktA, ppsA and yddG, p15A ori with PRPL;kanR | This study |
| pAP32 | pAP with aroGfbr, tyrAfbr and fpk, p15A ori with PRPL;kanR | This study |
| pAP33 | pAP with aroGfbr, tyrAfbr, fpk and pta, p15A ori with PRPL;kanR | This study |