Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 15;623(7989):1001–1008. doi: 10.1038/s41586-023-06743-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 5 — (a) Alignment of porcine OAS1, D. erecta cGLR, and bacterial CdnE03s. Conserved residues are highlighted in blue. (b) Alignment of the N-terminal helix, PβCD and C-terminal domains CD-NTases from staphylococcal species, belonging to different families (in different colors). The conserved basic residues involved in cabRNA binding by Ssc-CdnE03 are highlighted in red. Accession numbers are: S. nepalensis WP_096808822.1; S. hominis WP_0494417910.1; S. epidermidis WP_020363757.1 (CdnB03); S. pseudintermedius WP_101458501.1; S. saprophyticus WP_041080732.1; S. argenteus WP_076688059.1; S. hominis WP_071561002.1; S. sp. EGD-HP3 WP_021459889.1; S. haemolyticus WP_037559004.1; S. equorum WP_071664859.1; S. vitulinus WP_016911376.1; S. epidermidis W23144 (Cd–E03) - EES35648.1; S. sp. HMSC077B09 WP_070485035.1; S. schleiferi WP_050329628.1; S. chromogenes WP_037576868.1. (c) Detection of phage propagation after spotting ten-fold dilutions of the lytic DNA phages, Φ80α-vir, ΦNM1γ6, ΦNM4γ4, and Φ12γ3 onto lawns of S. aureus RN4220 harboring a plasmid expressing an incomplete (Sha-CdnE01 alone) or intact Sha-CBASS operon. (d) Agarose gel electrophoresis of the input and output RNA obtained after incubation of Sha-CdnE01 with total RNA extracted from uninfected staphylococci (RN4220) or from cells infected with Φ80α-vir phage. Data are representative of three independent experiments.