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. 2023 Nov 15;623(7989):1001–1008. doi: 10.1038/s41586-023-06743-9

Fig. 2. Secondary structures within the cabRNA are required for Ssc-CdnE03 activation.

Fig. 2

a, Agarose gel electrophoresis of pulled-down cabRNA treated with RNases T1 or III, or DNase (D). A ssRNA oligonucleotide was digested with RNase T1 as a control. Data are representative of three independent experiments. b, Thin-layer chromatography analysis of Ssc-CdnE03 products in the presence of total RNA extracted from infected cells and treated with RNases III, A, T1 or untreated. Data are representative of three independent experiments. c, Agarose gel electrophoresis of IVT cabRNA, unfolded (left) and folded (right), untreated or treated with RNase T1. Data are representative of three independent experiments. d, As in b, but incubating the cyclase with pulled-down, unfolded or folded IVT cabRNA.