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. 2023 Nov 22;623(7989):1026–1033. doi: 10.1038/s41586-023-06762-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a A representative tomogram showing immature HIV-1_BaL particles and MLV-CD4 VLPs. HIV-1 capsids are noted in green and MLV capsids are noted in purple. Scale bar = 50 nm. b Three-dimensional representation of immature HIV-1BaL particles and MLV-CD4 VLPs in a representative tomogram. Pink arrows represent Env trimers at membrane-membrane interfaces. Yellow arrows represent free Env trimers on the surface of the HIV-1BaL particles. c The number of Env trimers present at membrane-membrane interfaces for mature and immature HIV-1_BaL particles mixed with MLV-CD4 VLPs. Mature HIV-1_BaL mean ± s.d. = 7.5 ± 4. Immature HIV-1_BaL mean ± s.d. = 4.6 ± 2.5. P-value **** = <0.0001. d The distance between membranes at membrane-membrane interfaces with mature and immature HIV-1_BaL particles mixed with MLV-CD4 VLPs. Mature HIV-1_BaL mean ± s.d. = 14.3 nm ± 3.8 nm. Immature HIV-1_BaL mean ± s.d. = 13.5 nm ± 4.6 nm. * = 0.0420.