Extended Data Fig. 3. HIV-1 Env clustering is unaffected by capsid maturation in HIV-1 particles featuring a high number of Env trimers lacking the C-tail.
a Representative images of the HIV-1 viral particle produced from the cell line expressing EnvADA.CM.755*. The capsid is highlighted in green. Scale bar = 25 nm. b Representative images of the MLV VLP carrying CD4. The capsid is highlighted in purple. Scale bar = 25 nm. c A representative image of membrane-membrane interfaces in cryo-tomograms. HIV-1 and MLV capsids are highlighted in green and purple, respectively. Scale bar = 50 nm. d Violin plot of the number of Env trimers present at membrane-membrane interfaces for HIV-1BaL (blue), mature HIV-1ADA.CM.755* (brown), and immature HIV-1ADA.CM.755* (beige) with MLV-CD4 VLPs. P-values ns = 0.0779, ** = 0.0028, **** = <0.0001.