Extended Data Fig. 4. Plugging and engulfment depends on wetting and protein concentration.
a, Amount of exchanged solution, pore lifetime and budding vesicle fraction for the three main cases of interest (see Fig. 2i) at high (left) and low (right) protein-to-lumen concentration ratio ( and respectively) as a function of initial pore radius. b, Amount of exchanged solution, pore lifetime and budding vesicle fraction as a function of the wetting interaction strength for LLPS protein-solute interactions for high and low protein-to-lumen ratio (initial pore radius rp = 140 nm). At low protein concentrations the plugging effect is inhibited, and wetting has little effect. At high protein concentrations wetting promotes pore closure and can drive engulfment and budding of the droplet. Dots are the average over 10 replicas and error bars represent the standard deviation; τ is the MD unit of time.