Extended Data Fig. 3. Dietary CVA has no significant effect on CD8+ T cell function in tumor-bearing mice.
a, With CVA diet, TVA levels in B16F10 tumor-bearing mice serum (left) were measured by 1H nuclear magnetic resonance (NMR) spectroscopy (n = 4). Quantification of the percentage of CD8+ T cells among spleen, dLN, and intratumoral CD45+ cells (n = 5) (middle). Quantification of PD-1 expression among CD8+ T cells in spleen, dLN, and tumor (n = 5) (right). b, Quantification of LAG-3 expression among CD8+ T cells in spleen, dLN, and tumor (n = 5). c, Quantification of Ki-67- (left), ICOS- (middle), and GZMB-positive cells (right) among intratumoral CD8+ T cells (n = 5). d, Flow cytometry-based quantification of IL-2, TNF-α, or INF-γ-positive cells among intratumoral CD8+ T cells after in vitro phorbol myristate acetate (PMA)/ionomycin stimulation (n = 5, left three), flow cytometry and quantification of TCF1 and TOX expression among intratumoral CD8+ T cells (n = 5, right two). e, Mouse primary CD8+ T cells were isolated, activated, and treated with CVA, followed by analysis of CVA effect on cell functions. Relative cell number (n = 5), Ki-67-positive cells (n = 3), percentage of apoptotic cells (n = 6), relative TNF-α- (n = 6) or IFN-γ-positive cells (n = 5), Bcl2 level, and active caspase-3 level among CD8+ T cells (n = 6) were shown. Data are mean ± SD (a-e). Student’s two-sided unpaired t test (a-e).