Table 2.
Thermodynamic parameters calculated for the wt FTN2 and FTN2Azk variants based on DSC measurements. For wt FTN2 and ELC143Z, data were calculated from 3 or 2 DSC replicates. Tm (midpoint transient temperature), ΔH (unfolding enthalpy), ΔEa (activation energy), (KTm denaturation rate). Purified wt FTN2 and FTN2Azk variants were used for DSC analyses.
| Variants | Tm (°C) | ΔH (Kcal/mol) | ΔEa (Kcal/mol) | KTm (min−1) |
|---|---|---|---|---|
| Wild type FTN2 | 84.33 ± 0.09 | 402.6 ± 2.4 | 141.5 ± 2.6 | 0.56 ± 0.01 |
| ELC143Z | 84.2 ± 0.23 | 389.35 ± 0.99 | 138.1 ± 0.7 | 0.54 ± 0.00 |
| EHC89Z | 80.3 | 342.6 | 170.6 | 0.69 |