Fig. 2.

(A) Measurement of MitoP/MitoB following delivery of MitoB by injection into the posterior chamber of the eyes of C57Bl6/J mice. Mice were injected with 500 pmol MitoB in 1 μl PBS per eye and sacrificed 9 h post injection. Eyes were enucleated and snap-frozen until further analysis. Data are presented as individual measurements as well as means ± SEM. (B) MitoP/MitoB measurement following hyperoxia. C57Bl6/J pups together with their dams were placed under 75% oxygen (P7 pups) or at 85% O₂ (P8 pups). MitoB was injected intravitreally into the pups' eyes before they were placed under hyperoxia for 9 h before being sacrificed, eyes removed and snap frozen for MitoP and MitoB extraction. Some p8 pups and dams were placed at 85% for 3 days, with the dams rested at room air for 2 h per day. After 3 days the pups were removed and their eyes were injected with MitoB as above, and then placed back under hyperoxia for a further 9 h before being sacrificed, eyes removed and snap frozen for MitoP and MitoB extraction. Data are presented as individual measurements as well as means ± SEM. An unpaired two-tailed Student's t-test was used to identify differences between groups. *p < 0.05, ****p < 0.0001.