Figure 5.

xRho-L57R, but not xRho-V61R, results in inner segment mislocalization.A, image of xRho-M57R and B, xRho-V61R expressing retinas. Left panels: transillumination images; right panels: maximum projection confocal images. C, box-whisker plot with bee-swarm overlay of mislocalization indices, as described in Figure 1I. D–F, mutations of the other amino acids predicted to form hydrophobic interactions. Left and right panels as in A and B. G, box-whisker plot with bee-swarm overlay of mislocalization indices, as described in Figure 1I. The asterisks (∗) in C and G indicate significant difference at p = 0.05. H–L, mislocalization indices plotted as a function of expression level, as described in Figure 1J. Note that the dependence of mislocalization index (F_IS/(F_Is + F_OS)) on expression did not reach significance for any of the constructs. xRho, Xenopus rhodopsin.