Table 1.
IC50 (μM) for imipramine inhibition at +50 mV for WT and indicated mutant EAG1 channels
| Channel | IC50 (μM) | p Value |
|---|---|---|
| WT | 58.1 ± 9.7 (5) | |
| ΔPAS | 108.6 ± 12.6 (5)a | 0.0131 |
| Y71G | 26.7 ± 3.2 (4)a | 0.0279 |
| Y71V | 31.3 ± 1.8 (6)a | 0.0153 |
| Y71F | 58.0 ± 3.7 (6) | 0.9920 |
| Y71E | 28.5 ± 1.4 (5)a | 0.0166 |
| Y71R | 43.4 ± 7.5 (4) | 0.2892 |
| D39G | 79.6 ± 5.4 (6) | 0.0731 |
| V80G | 57.1 ± 3.1 (6) | 0.9176 |
| V83G | 44.3 ± 2.6 (6) | 0.1685 |
| R84G | 92.6 ± 2.7 (6)a | 0.0047 |
| F87G | 42.8 ± 0.6 (5) | 0.1541 |
| F130G | 52.8 ± 5.0 (6) | 0.6211 |
| D39G/R84G | 142 ± 28.5 (6)a | 0.0060 |
a
p < 0.05 by Student’s t test. p < 0.05 was considered statistically significant. p-values represent significance of Student’s t tests used to compare the IC50 for imipramine inhibition at +50 mV for WT and indicated mutant channels. The number of averaged recordings from different oocytes is indicated in parentheses. The difference between IC50 for ΔPAS and R84G mutants was not statistically significant (p value of 0.2067). The difference between IC50 for ΔPAS and D39G/R84G mutants was not statistically significant (p value of 0.0718).