Figure 4.
Regulation of the PPP by cyclin D1. Hepatocytes were cultured as in Figure 3. A, the abundance of unlabeled and fully 13C labeled (M6 or M5) PPP metabolites as determined by IC-UHR FTMS. B, Western blot of G6PD. C, G6PD enzyme activity assay using a commercial kit. G6PD, glucose-6-phosphate dehydrogenase; IC-UHR FTMS, ion chromatography coupled with ultra high-resolution Fourier transform mass spectrometry; PPP, pentose phosphate pathway.