Figure 7.

Effect of Cdk4 and DHODH inhibition in HCC cells. HuH7 cells were cultured in the presence of vehicle, palbociclib, and BAY2402234 as indicated. A, DNA synthesis as determined by BrdU uptake in cells treated with palbociclib (50 nM) and/or BAY2402234 (5 nM). B, Western blot of HuH7 cells cultured as in panel A. C, viability of cells treated with BAY2402234 at the indicated concentrations with and without palbociclib (5 μM, left), or with palbociclib at the indicated concentrations with and without BAY2402234 (5 nM, right). Cdk4, cyclin-dependent kinase 4; DHODH, dihydroorotate dehydrogenase; HCC, hepatocellular carcinoma.