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. 2023 Nov 16;14:1252174. doi: 10.3389/fphar.2023.1252174

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Copyright © 2023 Khajah, Hawai, Barakat, Albaloushi, Alkharji and Masocha.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of therapeutic treatment with the minocycline and methylprednisolone combination (MMP) regimen on the blood and colonic gene expression of pro- and anti-inflammatory cytokines The relative mRNA expression of the cytokines Il1b and Tnf in the blood both at (A) day 3 and (B) day 7 in untreated, DSS/vehicle (V)-treated, and DSS/minocycline plus methyl prednisolone (Mino + MP)-treated mice. Histobars represent the median plus the interquartile range of 3–5 mice in each group. The relative mRNA expression of the cytokines Il1b, Tnf, and Il10 in the colon both at (C) days 3 and (D) 7 in UT, V-treated, and Mino + MP-treated mice. Histobars represent the median plus the interquartile range of 7–19 mice in each group. * denotes a significant difference from UT mice (p < 0.05); ## denotes a significant difference from DSS/vehicle-treated mice (p < 0.01).