FIG. 1.

Lysis genes of Cp-1. (A) Localizations of ORF21 and ORF22, which encode holin and lysozyme, respectively. PL8 and PL9, represented by carets, are the late tandem promoters preceding the holin gene and are named according to the nomenclature given to the Cp-1 genome (14). (B) Amino acid sequence, translational start region, and predicted secondary structure of the Cph1 holin. RBS, ribosome-binding site. Filled and open bars represent putative transmembrane domains and β-turns, respectively. Positive (+) and negative (−) charges of amino acids are indicated above the sequence. Asterisks indicate the charged C-terminal domain. (C) Modular organization of Cpl1 lysozyme. The hatched region represents the N-terminal domain, and the six dotted boxes, P1 to P6, indicate choline-binding domains.