FIG. 2.

Schematic representation of the construction of pAMR11 and pAMR12. Plasmids are drawn with the relevant elements and restriction sites indicated. Thin and thick lines represent vector-derived sequences and Cp-1-derived sequences, respectively. Arrows represent the direction of transcription of the genes. Pm indicates an inducible promoter of the TOL plasmid, and xylS encodes the cognate regulator of Pm. Oligonucleotides used for isolation of the genes by PCR are marked as bent arrows. Sm and Km indicate the genes conferring streptomycin and kanamycin resistance, respectively, and RBS indicates the ribosome-binding sites.