FIG. 5.

Schematic representation of the construction of pAMR21 and pAMR22. Plasmids are drawn with the relevant elements and restriction sites indicated. Thin lines represent vector-derived sequences, and thick lines represent Cp-1-derived sequences. Arrows indicate the direction of transcription of the genes. P represents the promoter of the tetracycline resistance gene. Oligonucleotides used for isolation of the genes by PCR are indicated as bent arrows. Tc and Ery indicate the genes conferring tetracycline and erythromycin resistance, respectively, and RBS indicates the ribosome-binding sites.