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In vitro transcription-translation of plasmids pLSE1, pAMR21, and pAMR22. Samples containing pAMR22 (lane 1), pLSE1 (lane 2), and pAMR21 (lane 3) were loaded onto a sodium dodecyl sulfate–15% polyacrylamide gel. Arrows indicate the positions of the holin (Hol) and the lysozyme (Lys). Standard size markers, in kilodaltons, are shown on the left.
