TABLE 1.
Complementation of the function of the lambda Sam7 gene by the cph1 gene product
| E. coli strain | Gene(s) of interest | Phage lambda Sam7a
|
|
|---|---|---|---|
| With 3-MBb | Without 3-MB | ||
| LE392 | supF58 | + | + |
| HB101 | − | − | |
| HB101(pCIP100) | cpl1 | − | − |
| HB101(pAMR11) | cph1 | + | − |
| HB101(pAMR12) | cph1 and cpl1 | + | − |
a
The number of PFU per 100 μl of lambda phage was determined as described in Materials and Methods. + and − indicate that the ratio of plaques obtained for a particular strain and for the reference strain E. coli LE392 was >0.5:1 and <10−6:1, respectively.
b
3-MB was added to a final concentration of 0.2 mM.