Fig. 6.

Graft compatibility based on anatomical vascular reconnection is restricted to intrageneric combinations. Confocal micrographs of all surviving graft combinations at 30 DAG. Full junctions for each combination are shown above ×3 magnified panels that provide anatomical detail. Tomato:tomato (A, E), eggplant:tomato (B, F), pepper:tomato (C, G), groundcherry:tomato (D, H), tomato:eggplant (I, M), eggplant:eggplant (J, N), pepper:eggplant (K, O), tomato:pepper (O, S), eggplant:pepper (P,T), pepper:pepper (Q, U), groundcherry:pepper (R, V), tomato:groundcherry (W, AA), eggplant:grounchcherry (X, AB), pepper:groundcherry (Y, AC), groundcherry:groundcherry (Z, AD). Groundcherry:eggplant grafts exhibited insufficient survival to be imaged with statistically relevant replication. The y-axis of the image matrix displays the four stocks: Solanum lycopersicum (tomato), S. melongena (eggplant), Capsicum annuum (pepper), and Physalis pubescence (groundcherry). The x-axis shows the scion genotypes. The phylogenetic relationship of the scions is shown along the x-axis above the scions (Särkinen et al., 2013). Panels outlined with green boxes indicate grafts with successful vascular bridges, and panels outlined with white boxes indicate grafts with failed vascular connections. Yellow arrows point to xylem bridges. Grafts were harvested, stained with propidium iodide, and cleared in methyl salicylate prior to imaging. (A–D, I–K, O–R, and W–Z) Scale bars=1 mm; (E–H, L–N, S–V, and AA–AD) scale bars=333 µm. Additional replicates are included in Supplementary Fig. S3.