FIG. 2.

Genetic organization of the mecA regions of S. sciuri (K1), S. sciuri subsp. rodentius (K8 and K3), S. sciuri subsp. carnaticum (K11), and S. aureus. The S. aureus mecA region (c) was assembled by using DNA sequences from the database. The direction of gene transcription is shown by the arrows. Identical symbols are used to indicate sequences of significant similarity. Dashed symbols indicate regions determined by PCR analysis. Homologies between short DNA segments are indicated by • (near ORF454 of K3 and near ORF142 of S. aureus) or by •• (near CTORF225 of K3 and near CTORF168 of S. aureus). Accession numbers for these sequences are as follows: K8 (5,596 bp), Y13096; K3 mecA2 (6,368 bp), Y13095; K11 (6,684 bp), Y13094; K3 mecA1 (5,806 bp), Y13052; and K1 (5,068 bp), Y09223. To facilitate comparison, relevant DNA sequences of the mecA region of S. aureus are also provided: mecRI (1) (accession no. L14020), mecA (23) (accession no. X52593), hypervariable region (22) (accession no. X52594), and IS431 (4, 5) (accession no. X53818). These sequences were assembled to provide a map for the entire S. aureus mecA and flanking region (c [9,047 bp; accession no. Y14051]). Overlaps or gaps at junctions in this map were determined by sequencing the appropriate PCR products of S. aureus BB589 (7).