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. 1998 Jan;180(2):243–249. doi: 10.1128/jb.180.2.243-249.1998

TABLE 1.

Purification of the A. niger α-glucuronidase

Fraction Protein (mg) Total activity (μmol/min) Sp act (μmol/min/mg of protein) Purification factor Yield (%)
Pektinase 146 2,740 380 0.14 1 100
45% (NH4)2SO4 1,899 300 0.16 1.1 79
Phenyl Sepharose 619 195 0.31 2.2 51
Q-Sepharose 88 162 1.83 13.1 42
Superdex 200 PG 57 102 1.80 12.9 27
Mono Q 11 69 6.20 44.3 18
Butyl Sepharose 2.2 54 24.3 175 14.2
Poros 10 HQ 0.42 22 52.3 371 5.8