TABLE 1.
Fraction | Protein (mg) | Total activity (μmol/min) | Sp act (μmol/min/mg of protein) | Purification factor | Yield (%) |
---|---|---|---|---|---|
Pektinase 146 | 2,740 | 380 | 0.14 | 1 | 100 |
45% (NH4)2SO4 | 1,899 | 300 | 0.16 | 1.1 | 79 |
Phenyl Sepharose | 619 | 195 | 0.31 | 2.2 | 51 |
Q-Sepharose | 88 | 162 | 1.83 | 13.1 | 42 |
Superdex 200 PG | 57 | 102 | 1.80 | 12.9 | 27 |
Mono Q | 11 | 69 | 6.20 | 44.3 | 18 |
Butyl Sepharose | 2.2 | 54 | 24.3 | 175 | 14.2 |
Poros 10 HQ | 0.42 | 22 | 52.3 | 371 | 5.8 |