TABLE 2.

Synergistic effects of α-glucuronidase, endoxylanase, and β-xylosidase activity on release of 4-O-methylglucuronic acid, xylose, and xylo-oligomers from birchwood xylan

Enzyme(s) used in reaction mixtures	Amt released (μg/ml)a
	4-O-Methylglucuronic acid	Xylose	Xylobiose	Xylotriose
None (control)	63	0	0	0
α-Glucuronidase	62	0	0	0
Xylanase complex	127	1,260	440	129
Xylanase A	95	1,300	<50	<50
β-Xylosidase	63	270	0	0
α-Glucuronidase plus:
Xylanase complex	379	1,210	680	210
Xylanase A	206	1,360	60	70
β-Xylosidase	187	1,060	<50	<50
Xylanase A + β-xylosidase	107	1,320	<50	<50
Xylanase complex + β-xylosidase	146	1,410	<50	<50
α-Glucuronidase + xylanase A + β-xylosidase	398	1,500	<50	<50

^aThe amount of 4-O-methylglucuronic acid was determined by the colorimetric method. The amounts of the other compounds were determined by HPLC analysis.