TABLE 3.
Comparison of the number and average length of de novo assembled viral contigs obtained for VANA and dsRNA data sets normalized at different sequencing depths (100K, 300K, 1M, 3M, and 10M reads, five resampling repeats at each depth) a
| VANA average ± SD | dsRNA average ± SD | Two sample t-test | ||
|---|---|---|---|---|
| 100K reads | nb viral contigs | 33.6 ± 1.9 | 101.8 ± 2.9 | 9.2E-11 |
| Viral contigs average length | 733.4 ± 23.7 | 747.4 ± 17.1 | 0.32 | |
| 300K reads | nb viral contigs | 70.2 ± 5.4 | 129.4 ± 8.1 | 8.0E-07 |
| Viral contigs average length | 643.4 ± 27.8 | 887.8 ± 38.2 | 2.8E-06 | |
| 1M reads | nb viral contigs | 106.2 ± 6.3 | 159.2 ± 6.6 | 1.1E-06 |
| Viral contigs average length | 694.8 ± 30.3 | 1019.6 ± 40.9 | 5.7E-07 | |
| 3M reads | nb viral contigs | 129.6 ± 4.8 | 207.6 ± 3.8 | 2.5E-09 |
| Viral contigs average length | 798.4 ± 15.9 | 1067.6 ± 11.5 | 1.4E-09 | |
| 10M reads | nb viral contigs | 201.2 ± 4.1 | 268 ± 2.9 | 1.8E-09 |
| Viral contigs average length | 791.2 ± 11.1 | 1121.4 ± 10.6 | 3.9E-11 |
a
The SDs and the statistical differences (P-values) are also shown.