Fig 1.

B175L negatively regulates antiviral immune responses in stable PAM cells. PAM cells stably expressing B175L-Flag or a control plasmid were infected with ADV-GFP, HSV-GFP, or VACV-GFP (multiplicity of infection (MOI) = 1.0). The GFP images were captured at 24 hpi using fluorescence microscopy and quantified at 12 and 24 hpi using the fluorescence modulator. Virus titers of each sample were determined by standard plaque assay in A549 and Vero cells (A, C, and E). Porcine IFN-β and IL-6 concentrations in the cell culture supernatant collected at 12 hpi and 24 hpi were estimated by enzyme-linked immunosorbent assay (ELISA) (B, D, and F). (G and H) GFP images, fluorescence level, virus titer, IFN-β, and IL-6 ELISA from HSV-GFP-infected stable PIB cells. The data represent at least two independent experiments with similar results, and the values are expressed as means and SD for three biological replicates. Student’s t-test: *P  <  0.05; **P  <  0.01; ***P  <  0.001; ****P  <  0.0001; ns, not significant.