Fig 4.

Functional in vitro characterization of ADCC and ADCP Fc-effector mechanisms mediated by anti-M1 and anti-NP mAbs. In vitro ADCC activity of anti-M1 and anti-NP mAbs against A/Netherlands/602/2009 (H1N1) (A), A/Hong Kong/4801/2014 (H3N2) (B), and B/Colorado/06/2017 (C). In vitro ADCP activity of anti-M1 and anti-NP mAbs against A/Netherlands/602/2009 (H1N1) (D), A/Hong Kong/4801/2014 (H3N2) (E), and B/Colorado/06/2017 (F). CR9114 functioned as a positive control, whereas the anti-CHIKV E1 mAb (1D04) served as a negative control. Data are presented as areas under the curve (AUCs) calculated with a lower detection limit of the average background plus three standard deviations (SDs). Every symbol represents the AUC of a single mAb and bars denote mean ± SD of one experiment conducted with two technical replicates. For statistical significance calculations, values were first log(y) transformed, and subsequently, a one-way ANOVA corrected for multiple comparison test was performed. Only P values lower than 0.05 are shown.