Fig 5.

Effects of HERPUD1 and ERS agonists and inhibitors on PEDV replication and DHA/EPA effect. (A–D) Vero cells were infected with PEDV to control plasmid and siRNA for HERPUD1 treated with DHA or EPA, cells were collected at 24  h, and the expression levels of PEDV-N and ERS-related mRNAs were analyzed by qRT-PCR. (E) Vero cells were treated with 0–50 μM 4-PBA for 24 h. The relative cell viability was evaluated by the CCK-8 kit according to the manufacturer’s instructions. (F–I) PEDV-infected vero cells treated with DHA, EPA, and 4-PBA were collected at 24 h. Expression levels of PEDV-N and ERS-related mRNAs were analyzed by qRT-PCR and western blot. Results were presented as mean ± SD, and the asterisks indicate significance relative to controls (Student’s t test, *P < 0.05; **P < 0.01).