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. 2023 Nov 22;13(11):230019. doi: 10.1098/rsob.230019

Figure 4.

Figure 4.

Observing the effect of electromagnetic stimulation on homologues of EPG from different species. (a) Alignment of amino acid sequences for EPG and homologues B.g., BNCR, and CD59. (b,c) HeLa cells expressing Halo-N-B.g. and labelled with AF488 (b) and JFX650 (c) demonstrating this protein is membrane-associated. (d) Average intensity of GCaMP6m in HeLa cells expressing Halo-N-B.g. with various stimuli. (e,f) HeLa cells expressing Halo-N-BNCR and labelled with AF488 (e) and JFX650 (f) showing the protein, unexpectedly, does not localize to the membrane. (g) Average intensity of GCaMP6m in HeLa cells also expressing Halo-N-BNCR with various stimuli. (h,i) HeLa cells expressing Halo-N-CD59 labelled with AF488 (h) and JFX650 (i) demonstrating this protein is membrane-associated. (j) Average intensity of GCaMP6m in HeLa cells also expressing Halo-N-CD59 with various stimuli. (d,g,j) Error bars are representative of 95% CI. All scale bars indicate 50 μm. The Halo-N-B.g. group included n = 84, n = 97, and n = 91 cells over 3 experiments for no stimulus, sham, and active groups respectively. The Halo-N-BNCR group included n = 118, n = 120, and n = 105 cells over 3 experiments for no stimulus, sham, and active groups respectively. The Halo-N-CD59 group included n = 130, n = 124, and n = 120 cells over 4 experiments for the no stimulus, sham, and active groups respectively.