FIG. 4.
Alignment of the deduced amino acid sequences of the five V. cholerae flagellin proteins. The sequences were aligned by Pileup (GCG Inc.). Amino acids which are identical in at least three of the flagellin genes are capitalized; amino acids which are identical in all five flagellin genes are denoted by an asterisk. The sequences used to design degenerate oligonucleotides for PCR are underlined (FLAX12; see Materials and Methods).