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. 1998 Jan;180(2):303–316. doi: 10.1128/jb.180.2.303-316.1998

TABLE 3.

All five flagellin promoters are transcribed simultaneously in V. choleraea

Genotypeb β-Galactosidase activity in strain with mutation:
flaAp-′lacZ flaCp-′lacZ flaEp-′lacZ flaDp-′lacZ flaBp-′lacZ
Wild type 9,641 11,047 3,417 14,639 16,298
ΔflaA::Cmr 11,063 13,789 6,833 11,341 15,833
ΔtoxR 9,436 15,441 5,101 13,846 13,407
a

Assays were performed as described in Materials and Methods. Strains were grown in LB supplemented with 2 mM glutamine; cultures were assayed in triplicate at an optical density at 600 nm of ∼0.2 to 0.4. Results are the average of three samples expressed in Miller units (38). Each strain harboring the vector pRS551 alone grown in this medium has 10 to 15 Miller units of activity, which can be considered background activity. 

b

The actual strains used (Table 1) were CG842 (wild type), KKV90 (ΔflaA::Cmr), and KKV62 (ΔtoxR), harboring plasmids pKEK80 (flaAp-′lacZ), pKEK76 (flaCp-′lacZ), pKEK81 (flaEp-′lacZ), pKEK77 (flaDp-′lacZ), and pKEK79 (flaBp-′lacZ). 

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