TABLE 1.

Sequences of the H. pylori CopA ATPase inserted into vector M0 or M1^a

Inserts	Sequence	Positions	Glycosylationb		Activityc
			M0	M1
H1	LALAVIFTLFVVYLSMGAMLS	L84 to S104	+	−	SA, ST
H2	HSNFLNACLQLIGTLIVMHLGR	H118 to R139	±	−	SA, ST
H3	LIAIGTSAALISSLWQLYLVYT	L159 to T180	+	−	SA, ST
H4	WSYGHYYFESVCVILMFVMVGR	W183 to R205	+	−	SA, ST
HXa	LVDSIVVGDILKVLPGSAIAV	L241 to V261	−	+
HXb	NQQIEVLVDSIVVGDILKVLPGSAIAVDG	N235 to G263	−	+
H5	VSSVFVPSVIAIAILAFVVWLIIAA	V340 to A363	+	−	SA, ST
H6a	FWWNFGIALEVFVSVLVISCPCALGLATPMSIL	F368 to L400	±	−	SA, ST
H6b	FWWNFGIALEVFVSVLVISCPCALGLA	F368 to A394	±	−	SA, ST
H6c	FWWNFGIALEVFVSVLVI	F368 to I385	±	−	SA, ST
H6d	IALEVFVSVLVISCPCALGLATPMSIL	I374 to L400	±	−	SA, ST
H6e	IALEVFVSVLVISCPCALGLA	I374 to A394	−	−	ST
H6f	IALEVFVSVLVIS	I374 to I385	±	−	SA, ST
HY	LNDAPSLAMSDVAVVMK	L629 to K646	−	+
H7	NLFWAFCYNSVFIPLACGVL	N684 to L703	−	−	ST
H7a	KENLFWAFCYNSVFIPLACGVLKA	K682 to K705	−	−	ST
H7b	KENLFWAFCYNSVFIPLACGVL	K682 to L705	−	−	ST
H7c	KNIKENLFWAFCYNSVFIPLACGVL	K679 to L703	−	−	ST
H7d	KNIKENLFWAFCYNSVFIPLACGVLKA	K679 to K705	−	−	ST
H7e	NLFWAFCYNSVFIPLACGVLKA	N684 to K705	−	−	ST
H8a	IMLSPAIAGLAMSLSSVSVVL	I708 to K728	−	−	ST
H8b	IMLSPAIAGLAMSLSSVSVVLNSQRLRNFKIK	I708 to K739	−	−	ST
HY-7	LNDAPSLAMS/––––––––/VFIPLACGVL–	L629 to L703	±	ND	SAd
HY-8a	LNDAPSLAMS/––––––––/MSLSSVSVVL–	L629 to L728	−	ND	SAd
HY-8b	LNDAPSLAMS/––––––––/SQRLRNFKIK–	L629 to K739	−	ND	SAd

^aIn the fusion proteins, either HK-M0 or HK-M1 is followed by the listed copper ATPase insert sequences and the β-subunit segment. The last three columns present a summary of the results of all the different inserts expressed in the vectors HK-M0 (M0) and HK-M1 (M1). 

^b+, presence of glycosylation; −, absence of glycosylation; ±, variable glycosylation; ND, not determined. 

^cSA, signal anchor activity; ST, stop transfer activity. 

^dStop transfer activity not determined.