TABLE 1.
Inserts | Sequence | Positions | Glycosylationb
|
Activityc | |
---|---|---|---|---|---|
M0 | M1 | ||||
H1 | LALAVIFTLFVVYLSMGAMLS | L84 to S104 | + | − | SA, ST |
H2 | HSNFLNACLQLIGTLIVMHLGR | H118 to R139 | ± | − | SA, ST |
H3 | LIAIGTSAALISSLWQLYLVYT | L159 to T180 | + | − | SA, ST |
H4 | WSYGHYYFESVCVILMFVMVGR | W183 to R205 | + | − | SA, ST |
HXa | LVDSIVVGDILKVLPGSAIAV | L241 to V261 | − | + | |
HXb | NQQIEVLVDSIVVGDILKVLPGSAIAVDG | N235 to G263 | − | + | |
H5 | VSSVFVPSVIAIAILAFVVWLIIAA | V340 to A363 | + | − | SA, ST |
H6a | FWWNFGIALEVFVSVLVISCPCALGLATPMSIL | F368 to L400 | ± | − | SA, ST |
H6b | FWWNFGIALEVFVSVLVISCPCALGLA | F368 to A394 | ± | − | SA, ST |
H6c | FWWNFGIALEVFVSVLVI | F368 to I385 | ± | − | SA, ST |
H6d | IALEVFVSVLVISCPCALGLATPMSIL | I374 to L400 | ± | − | SA, ST |
H6e | IALEVFVSVLVISCPCALGLA | I374 to A394 | − | − | ST |
H6f | IALEVFVSVLVIS | I374 to I385 | ± | − | SA, ST |
HY | LNDAPSLAMSDVAVVMK | L629 to K646 | − | + | |
H7 | NLFWAFCYNSVFIPLACGVL | N684 to L703 | − | − | ST |
H7a | KENLFWAFCYNSVFIPLACGVLKA | K682 to K705 | − | − | ST |
H7b | KENLFWAFCYNSVFIPLACGVL | K682 to L705 | − | − | ST |
H7c | KNIKENLFWAFCYNSVFIPLACGVL | K679 to L703 | − | − | ST |
H7d | KNIKENLFWAFCYNSVFIPLACGVLKA | K679 to K705 | − | − | ST |
H7e | NLFWAFCYNSVFIPLACGVLKA | N684 to K705 | − | − | ST |
H8a | IMLSPAIAGLAMSLSSVSVVL | I708 to K728 | − | − | ST |
H8b | IMLSPAIAGLAMSLSSVSVVLNSQRLRNFKIK | I708 to K739 | − | − | ST |
HY-7 | LNDAPSLAMS/––––––––/VFIPLACGVL– | L629 to L703 | ± | ND | SAd |
HY-8a | LNDAPSLAMS/––––––––/MSLSSVSVVL– | L629 to L728 | − | ND | SAd |
HY-8b | LNDAPSLAMS/––––––––/SQRLRNFKIK– | L629 to K739 | − | ND | SAd |
In the fusion proteins, either HK-M0 or HK-M1 is followed by the listed copper ATPase insert sequences and the β-subunit segment. The last three columns present a summary of the results of all the different inserts expressed in the vectors HK-M0 (M0) and HK-M1 (M1).
+, presence of glycosylation; −, absence of glycosylation; ±, variable glycosylation; ND, not determined.
SA, signal anchor activity; ST, stop transfer activity.
Stop transfer activity not determined.