Figure 3. Profiling RNA polymerase II in isolated spermatocytes.
(A) An adult testis carrying a UASRFP construct induced by a bamGAL4 driver and a hephGFP construct. Gonial cells are labeled red while spermatocytes are labeled green with fluorescent proteins. (B) Fluorescence-activated cell sorting (FACS) plots of recorded events from dissociated testes for forward scatter (FSC) and GFP signal. Boxes indicate events collected for chromatin profiling. (C) Distribution of RNAPIIS2p at the spermatocyte-expressed betaTub85D gene in isolated spermatocytes and in wing imaginal discs. (D) Distribution of RNAPIIS2p at the broadly expressed stc gene in isolated spermatocytes and in wing imaginal discs. RNAPIIS2p is strongly localized at the stc promoter in wing imaginal discs, but more evenly distributed in spermatocytes. (E) Selected examples of genes with late germline expression and for protamines. Expression z-scores from FCA snRNA-seq across 18 germline clusters (Raz et al., 2023) and RNAPIIS2p enrichment in isolated spermatocytes. (F) Enrichment of RNAPIIS2p in isolated spermatocytes and in wing imaginal discs across genes with germline-enriched transcripts.
Figure 3—figure supplement 1. Distribution of elongating RNAPII in spermatocytes and in somatic cells.
