Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-H3-K4-dimethyl (rabbit monoclonal) |
Epicypher | Cat. No. 13–0027 RRID: AB_3068541 |
CUT&Tag (1:100) |
| Antibody | Anti-RNAPII-Serine-2-phosphorylation (rabbit monoclonal) |
Cell Signalling Technology | Cat. No. 13499 RRID: AB_2798238 |
CUT&Tag (1:100) |
| Antibody | Anti- H2A-K119-ubiquitinylation (rabbit monoclonal) |
Cell Signalling Technology | Cat. No. 8240 RRID: AB_10891618 |
CUT&Tag (1:100) IFF (1:100) |
| Antibody | Anti-H3-K9-dimethyl (mouse monoclonal) |
EMD Millipore | Cat. No. 05–1249 RRID: AB_11210998 |
CUT&Tag (1:100) |
| Antibody | Anti-H3-K27-trimethyl (rabbit monoclonal) |
Cell Signalling Technology | Cat. No. 9733 RRID: AB_2616029 |
CUT&Tag (1:100) |
| Antibody | Anti-H4-K16-acetyl (rabbit monoclonal) |
Abcam | Cat. No. ab109463 RRID: AB_10858987 |
CUT&Tag (1:100) |
| Antibody | ab5821 anti-fibrillarin (rabbit polyclonal) |
Abcam | Cat. No. ab5821 RRID: AB_2105785 |
IFF (1:100) |
| Genetic reagent (Drosophila melanogaster) | w1118 | Bloomington Drosophila Stock Center | BDSC:3605; RRID:BDSC_3605 | |
| Genetic reagent (D. melanogaster) | bamD86 | Bloomington Drosophila Stock Center | BDSC:5427; RRID:BDSC_5427 | |
| Genetic reagent (D. melanogaster) | Df(3R)FDD-0089346 | Bloomington Drosophila Stock Center | BDSC:27402; RRID:BDSC_27402 | Deficiency including bam |
| Genetic reagent (D. melanogaster) | aly1 | Bloomington Drosophila Stock Center | BDSC:1148; RRID:BDSC_1148 | |
| Genetic reagent (D. melanogaster) | Df(3L)BSC428 | Bloomington Drosophila Stock Center | BDSC:24932; RRID:BDSC_24932 | Deficiency including aly |
| Genetic reagent (D. melanogaster) | Heph-GFP | Bloomington Drosophila Stock Center | BDSC:51540; RRID:BDSC_51540 | P[PTT-GC]hephCC00664 |
| Genetic reagent (D. melanogaster) | bam-GAL4 | Bloomington Drosophila Stock Center | BDSC:80579; RRID:BDSC_80579 | P[bam-GAL4:VP16,w+]1 |
| Genetic reagent (D. melanogaster) | UAS-RFP | Bloomington Drosophila Stock Center | BDSC:30556; RRID:BDSC_30556 | P[UAS-RFP,w+]2 |
| Genetic reagent (D. melanogaster) | Pc-GFP | Bloomington Drosophila Stock Center | BDSC:9593; RRID:BDSC_9593 | P[Pc-eGFP,w+]3 |
| Biological sample (D. melanogaster) | Wing imaginal discs | This paper | Dissected tissue | |
| Biological sample (D. melanogaster) | Adult testes | This paper | Dissected tissue | |
| Biological sample (D. melanogaster) | Spermatocytes | This paper | Dissected tissue |