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. 2023 Dec 1;133(23):e172600. doi: 10.1172/JCI172600

Figure 6. Amounts of LPL in the postheparin plasma are lower in Apoa5–/– mice than in Apoa5+/+ mice.

Figure 6

Apoa5–/– and Apoa5+/+ mice were given an intravenous injection of heparin (500 U/kg), and plasma samples were collected 2 minutes later. (A and B) LPL mass and activity levels in the postheparin plasma of individual Apoa5–/– and Apoa5+/+ mice during ad libitum feeding (A) and during fasting (B). Apoa5–/– mice, n = 5 in A and n = 6 in B; Apoa5+/+ mice, n = 7 in A and n = 9 in B. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001, by unpaired, 2-tailed Student’s t test. (C–E) Amounts of LPL in postheparin plasma from Apoa5–/– and Apoa5+/+ mice (n = 3 mice/group), as assessed by HS chromatography. LPL appeared in the “high-salt” fractions (fractions 21–27; 1.13–1.38 M NaCl). (C) LPL mass and activity levels in the HS fractions from the postheparin plasma of Apoa5–/– and Apoa5+/+ mice during ad libitum feeding. (D) Western blots (WB) of LPL in fractions 21–27 from fasted Apoa5–/– and Apoa5+/+ mice. Band intensity was measured with an infrared scanner. (E) Levels of LPL mass and activity in fractions 21–27 from fasted Apoa5–/– and Apoa5+/+ mice. The reported results were confirmed in 2 independent experiments.