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. 2023 Dec 1;133(23):e172600. doi: 10.1172/JCI172600

Figure 8. IBA490, an inhibitory ANGPTL3/8-specific mAb, increases intracapillary LPL levels, relative to GPIHBP1 or CD31, in Apoa5–/– mice.

Figure 8

Apoa5–/– and Apoa5+/+ mice were given a subcutaneous injection of IBA490 or a control mAb (10 mg/kg). Twenty-four hours later, the mice were given an intravenous injection of Alexa Fluor–labeled mAbs against mLPL (27A7), GPIHBP1 (11A12), and CD31 (2H8). After 10 minutes, mice were euthanized and then perfused with PBS, and tissue sections were prepared for fluorescence microscopy. (A and B) Confocal micrographs of LPL, GPIHBP1, and CD31 staining along the luminal surface of capillaries in heart tissue (A) and BAT (B) of IBA490- or control mAb–treated Apoa5–/– and Apoa5+/+ mice. Scale bars: 50 μm. The amounts of intracapillary LPL relative to the amounts of intracapillary GPIHBP1 or CD31 were quantified in 3 independent experiments, and the data are shown in Supplemental Figure 7.