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. 2023 Dec 1;133(23):e161701. doi: 10.1172/JCI161701

Figure 6. Disruption of MD and WD development in E12.5 Osr1–/– embryos.

Figure 6

(A and B) MDs and WDs (black arrows) are both visualized bilaterally in the urogenital ridge (UGR) of an E12.5 Osr1+/– littermate control (A), but the MDs are absent and WDs are truncated on the left side of an E12.5 Osr1–/– mouse (B). (C–J) H&E-stained sections extending rostrally to caudally (R → C), and specific E-cadherin immunostaining (green) of the WDs at the same level of the urogenital ridge (UGR), showing that MDs are absent and WDs are rostrally truncated on the left side (D), and MDs are rostrally truncated on the right side (F), while WDs appear to have developed normally on the right side of E12.5 Osr1–/– mice (H and J). UGR, urogenital ridge; US, urogenital sinus; R, rostral, C, caudal. Scale bars: 100μm.