FIG. 4.

Immunoblot analysis of YopM, LcrV, and LcrQ expressed in the ysc mutant Y. pestis KIM5-3001.2 (ΔlcrD) and the double mutant Y. pestis KIM5-3001.2 (ΔlcrD ΔyopD). Bacteria were grown at 37°C in the absence (−) or presence (+) of 2.5 mM Ca²⁺. Proteins from equal numbers of cells (0.025 A₆₂₀ unit · ml) were separated by SDS-PAGE in a 12 (YopM and LcrV) or 15% (LcrQ) (wt/vol) polyacrylamide gel. Antibodies were used to detect YopM, LcrV, and LcrQ in both the whole-cell (C) (cytoplasmic, periplasmic, and membrane) fraction and the extracellular (E) fraction. The positions of the LCR-related proteins are indicated.