Skip to main content
. 2023 Sep 21;35(12):4325–4346. doi: 10.1093/plcell/koad239

Figure 8.

Figure 8.

SMG4 acts in a common pathway with CYP78As to regulate grain size. A and B) Firefly LCI assays shows that SMG4 interacts with GL3.2 (A) and BG2L1 (B) in N. benthamiana leaves cells. CL, C terminus of LUC; NL, N terminus of LUC. The scale bar indicates the luminescence intensity in CPS. C) In vivo Co-IP assay showing that SMG4 interacts with GL3.2 and BG2L1 in rice protoplasts. The symbols “+” and “–” represent the presence and absence of the corresponding proteins. D) Grain morphologies of CYP78A (BG2 + GL3.2 + BG2L1) RNAi lines in the kit and SMG4-OE#3 backgrounds. Scale bar, 5 mm. E–G) Grain length (n = 30) (D), grain width (n = 30) (E), and grain thickness (n = 30) (F) of kit and CYP78A (BG2 + GL3.2 + BG2L1) RNAi lines in the Kit and SMG4-OE#3 backgrounds. Values are means ± SD. Different letters indicate significant differences ranked by pairwise multiple comparison followed by Tukey's test (P < 0.05).