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. 2023 Nov 13;26(12):2104–2121. doi: 10.1038/s41593-023-01480-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Karyogram of E4-S/S-A, E4-S/S-B, E4-S/S-C, E4-R/S-A, and E4-R/S-B hiPSC lines. For all cell lines, metaphase was examined for n = 20 cells per line. b, Representative immunofluorescent images of pluripotent stem cell markers Nanog, OCT3/4, SOX2, hNuclei, and TRA-1-60 from E4-S/S-A, E4-S/S-B, E4-S/S-C, E4-R/S-A, and E4-R/S-B hiPSC lines (scale bar, 100 μm). c, Representative immunofluorescent images of mature neuronal marker MAP2 (green) and DAPI (blue) in 3-week-old neurons differentiated from E4-S/S-A, E4-S/S-B, E4-S/S-C, E4-R/S-A, and E4-R/S-B hiPSC lines (scale bar, 20 μm). Representative images were selected from n = 3 fields of view from each cell line.