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Western blot analysis of the expression of various uvrD alleles. Experiments were carried out as described in Materials and Methods. Lanes: 1, 75 ng of purified UvrD+ protein; 2 to 9, 15 μg of total cell protein from strains carrying various uvrD alleles as follows: 2, SK9052 (uvrD300); 3, SK9053 (uvrD301); 4, SK9054 (uvrD302); 5, SK9055 (uvrD303); 6, SK9056 (uvrD304); 7, SK9040 (uvrDΔ294::Kanr); 8, SK9041 (uvrD+); 9, SK707 (uvrD+).
