TABLE 2.
Plasmids used in this work
| Plasmid | Descriptiona | Source or reference |
|---|---|---|
| pGP1-3 | Tcr plasmid carrying T7 RNA polymerase gene under λcI857 control | 44 |
| pWSK29 | Apr low-copy-number vector | 47 |
| pGZK20 | Apr low-copy-number uvrD+ plasmid | uvrD+ cloned in SalI site of pWSK29 |
| pGZK28 | ApruvrD300 (A406T) substitution in pGZK20 | Isolated by EMS mutagenesis |
| pGZK29 | ApruvrD301 (A406V) substitution in pGZK20 | Site-directed mutagenesis |
| pGZK30 | ApruvrD302 (A406S) substitution in pGZK20 | Site-directed mutagenesis |
| pGZK31 | ApruvrD303 (D403AD404A) substitution in pGZK20 | Site-directed mutagenesis |
| pGZK32 | ApruvrD304 (E408AR409A) substitution in pGZK20 | Site-directed mutagenesis |
| pGZK33 | ApruvrD+ (T406A) substitution in pGZK28 | Site-directed mutagenesis |
a
Mutationally altered uvrD genes were isolated as described in Materials and Methods. The positions of amino acid substitutions are indicated in parentheses.