TABLE 3.
Mutation frequencies in various uvrD mutants
| Strain | Genotype | Mutation frequency (10−10)a | Relative mutabilityb |
|---|---|---|---|
| SK707 | uvrD+ | 1.9 ± 1.1 | 1.0 |
| SK9040 | uvrDΔ294/pWSK29 | 490 ± 230 | 258 |
| SK9041 | uvrDΔ294/pGZK20 (uvrD+) | 1.3 ± 0.6 | 0.7 |
| SK9052 | uvrDΔ294/pGZK28 (uvrD300) | 2.4 ± 0.1 | 1.3 |
| SK9053 | uvrDΔ294/pGZK29 (uvrD301) | 0.39 ± 0.05 | 0.2 |
| SK9054 | uvrDΔ294/pGZK30 (uvrD302) | 1.8 ± 0.4 | 1.0 |
| SK9055 | uvrDΔ294/pGZK31 (uvrD303) | 0.37 ± 0.02 | 0.2 |
| SK9056 | uvrDΔ294/pGZK32 (uvrD304) | 0.27 ± 0.13 | 0.1 |
a
Mutation frequencies for spectinomycin resistance were calculated by dividing the number of colonies growing on each spectinomycin plate by the number of total cells originally applied to the plate. The data are presented as the average of three independent experiments. All experiments were performed at 37°C as described in Materials and Methods. The viabilities of all the strains tested were identical within the experimental error.
b
Relative mutability was calculated by comparing the average mutation frequency with that of strain SK707 (uvrD+).