TABLE 4.
Conjugal recombination in various uvrD mutants
| Strains | Genotype | No. of his+ recombinants/ml | Relative recombination proficiencya |
|---|---|---|---|
| SK707 | uvrD+ | (5.1 ± 0.7) × 103 | 1.0 |
| SK9040 | uvrDΔ294/pWSK29 | (8.5 ± 0.7) × 104 | 16.9 |
| SK9041 | uvrDΔ294/pGZK20 (uvrD+) | (3.0 ± 0.7) × 103 | 0.6 |
| SK9052 | uvrDΔ294/pGZK28 (uvrD300) | (1.5 ± 0.1) × 103 | 0.3 |
| SK9053 | uvrDΔ294/pGZK29 (uvrD301) | (1.3 ± 0.3) × 103 | 0.3 |
| SK9054 | uvrDΔ294/pGZK30 (uvrD302) | (2.9 ± 0.5) × 103 | 0.6 |
| SK9055 | uvrDΔ294/pGZK31 (uvrD303) | (0.5 ± 0.4) × 103 | 0.1 |
| SK9056 | uvrDΔ294/pGZK32 (uvrD304) | (2.4 ± 0.7) × 103 | 0.5 |
a
Recombination assays were performed at 37°C as described in Materials and Methods. Relative recombination proficiency is determined as the recombination frequency divided by that of SK707 (uvrD+). Data represent the average of three independent experiments. The viabilities of all of the strains tested were identical within the experimental error.