TABLE 5.
Intrachromosomal recombination in various uvrD mutants
| Strain | Genotype | No. of lac+ recombinants/plate | Relative recombination proficiencya |
|---|---|---|---|
| SK707 | uvrD+ | 76 ± 10 | 1.0 |
| SK9040 | uvrDΔ294/pWSK29 | 502 ± 35 | 6.4 |
| SK9041 | uvrDΔ294/pGZK20 (uvrD+) | 96 ± 16 | 1.2 |
| SK9052 | uvrDΔ294/pGZK28 (uvrD300) | 70 ± 14 | 0.9 |
| SK9053 | uvrDΔ294/pGZK29 (uvrD301) | 84 ± 18 | 1.1 |
| SK9054 | uvrDΔ294/pGZK30 (uvrD302) | 108 ± 24 | 1.4 |
| SK9055 | uvrDΔ294/pGZK31 (uvrD303) | 42 ± 16 | 0.5 |
| SK9056 | uvrDΔ294/pGZK32 (uvrD304) | 50 ± 19 | 0.6 |
a
Recombination tests were performed as described in Materials and Methods. Equal amounts of cells for each strain were plated (approximately 107 cells/plate) on lactose minimal plates. All experiments were conducted at 37°C. The viabilities of all of the strains tested were identical within the experimental error.